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49000 Series

Chroma’s 49000 series of single band sets features our highest throughput sputter coatings (ET) which achieve the highest transmission levels for interference filters. These filters have very steep transitions from peak transmission to high optical density to best exploit the spectral properties of modern fluorochromes with short Stokes shifts. This is because steep transitions allow for very little spectral separation between excitation and emission bands.
These filters offer the greatest hardness and durability against environmental factors such as high heat and humidity, and are covered by Chroma’s lifetime warranty. All ET filters sets provide zero-pixel-shift image registration* between filter sets, at no additional cost.

*It is important to note that filters are only one factor in achieving perfect image registration in imaging systems. All other components and devices must also be in register.

  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • ET Series
  • White Light

49027

ET – Brilliant™ Violet 421, Alexa Fluor 405

Best with these fluorochromes
  • Brilliant Violet™ 421

This narrow-band filter set is intended for very bright fluorochromes such as BD Horizon Brilliant™ Violet 421. It is sufficiently narrow to allow spectral separation from cyan fluors such as BD Horizon Brilliant™ Violet 480, cyan fluorescent proteins and Aqua FISH probes. This set is also useful for detecting DAPI. DAPI will appear violet instead of blue.

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET395/25x (EX) $ 325.00
T412lpxt (BS) $ 225.00
ET431/28m (EM) $ 325.00
$825.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • ET Series
  • White Light

49025

ET - DAPI/FluoroGold Longpass

Best with these fluorochromes
  • FluoroGold

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
AT350/50x (EX) $ 150.00
T400lp (BS) $ 225.00
ET425lp (EM) $ 325.00
$625.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • ET Series
  • White Light

49021

ET - EBFP2/Coumarin/Attenuated DAPI

Best with these fluorochromes
  • DyLight 405
  • EBFP2
  • Pacific Blue
  • TagBFP

Excitation Intensity Note

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET405/20x (EX) $ 325.00
T425lpxr (BS) $ 225.00
ET460/50m (EM) $ 325.00
$825.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • FISH
  • ET Series
  • White Light

49028

ET – DAPI for 395nm light sources

Best with these fluorochromes
  • Atto 390
  • Biosearch Blue
  • DAPI
  • EBFP2
  • LysoTracker Blue/MeOH
  • TagBFP

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET395/25x (EX) $ 325.00
T425lpxr (BS) $ 225.00
ET460/50m (EM) $ 325.00
$825.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • ET Series
  • White Light
  • Multi LED Light Engines

49053

ET - DAPI for 355-405nm LEDs

Optimized for use with CoolLED light engines.
This set features a wide band excitation filter which is compatible with a broad range of LEDs with peaks between 355-405nm, intended for fluorophores such as DAPI. This set is intended for use with LEDs. If instead, it is used with a broad white light source such as a mercury or metal halide lamp, the excitation filter will transmit very high levels of light to the sample.

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET385/70x (EX) $ 325.00
T425lpxr (BS) $ 225.00
ET460/40m (EM) $ 325.00
$825.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • FISH
  • ET Series
  • White Light

49000

ET - DAPI

Best with these fluorochromes
  • Alexa Fluor 350™
  • Biosearch Blue
  • Coumarin
  • DAPI
  • DyLight 350
  • Hoechst 33258
  • LysoTracker Blue/MeOH

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
AT350/50x (EX) $ 150.00
T400lp (BS) $ 225.00
ET460/50m (EM) $ 325.00
$625.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • ET Series
  • White Light

49001

ET - ECFP

Best with these fluorochromes
  • AmCyan
  • Brilliant Violet™ 480
  • Cerulean
  • CFP
  • ECFP

Excitation Intensity Note

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET436/20x (EX) $ 325.00
T455lp (BS) $ 225.00
ET480/40m (EM) $ 325.00
$825.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • ET Series
  • White Light

49032

ET – Brilliant™ Violet 480

Best with these fluorochromes
  • Brilliant Violet™ 480

This narrow-band filter set is intended for very bright fluorochromes such as BD Horizon Brilliant™ Violet 480 (BV 480). When used with BV480, it is sufficiently narrow to allow spectral separation from green fluors such as Alexa fluor 488, FITC, GFP and green FISH probes, as well as separation from violet fluors such as Brilliant Violet™ 421.

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET436/20x (EX) $ 325.00
T455lp (BS) $ 225.00
ET480/30m (EM) $ 325.00
$825.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • ET Series
  • White Light

49013

ET - TFP (Teal FP)

Best with these fluorochromes
  • Atto 425
  • mTFP1

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET445/30x (EX) $ 325.00
T470lpxr (BS) $ 225.00
ET500/40m (EM) $ 325.00
$825.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • ET Series
  • White Light

49012

ET - FITC/EGFP Longpass

Best with these fluorochromes
  • Acridine Orange + DNA and RNA
  • JC-1 non-ratiometric

Excitation Intensity Note

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET480/40x (EX) $ 325.00
T510lpxrxt (BS) $ 275.00
ET510lp (EM) $ 325.00
$825.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • ET Series
  • White Light

49020

ET - Narrow Band EGFP to Minimize Autofluorescence

Best with these fluorochromes
  • Azami Green
  • DiO
  • EGFP
  • Emerald GFP
  • GFP
  • LysoTracker Green/pH 5.2
  • mWasabi
  • ZsGreen1

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET480/20x (EX) $ 325.00
T495lpxr (BS) $ 225.00
ET510/20m (EM) $ 325.00
$825.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • ET Series
  • White Light

49018

ET - EGFP Longpass

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET470/40x (EX) $ 325.00
T495lpxt (BS) $ 275.00
ET500lp (EM) $ 325.00
$825.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • ET Series
  • White Light

49029

ET - EYFP Longpass

Excitation Intensity Note

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET500/20x (EX) $ 325.00
T515lp (BS) $ 225.00
ET520LP (EM) $ 325.00
$825.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • ET Series
  • White Light
  • Multi LED Light Engines

49054

ET - GFP for 455-465nm LEDs

Optimized for use with CoolLED light engines.
This set features an excitation filter which is shifted to slightly shorter wavelengths to be most compatible with blue LEDs with peaks between 455-465nm. GFP will be brighter compared with more standard sets that transmit 470-490nm LEDs.

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET460/30x (EX) $ 325.00
T495lpxr (BS) $ 225.00
ET520/40m (EM) $ 325.00
$825.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • ET Series
  • White Light

49002

ET - EGFP (FITC/Cy2)

Best with these fluorochromes
  • Acridine Orange + DNA
  • Alexa Fluor 488™
  • Atto 465
  • Azami Green
  • BODIPY FL/pH7.2
  • Cy2™
  • DiO
  • DyLight 488
  • EGFP
  • Emerald GFP
  • FAM
  • FITC
  • Fluo-4
  • GFP
  • LysoTracker Green/pH 5.2
  • MitoTracker Green FM/MeOH
  • mWasabi
  • SYBR® Green I
  • SYTO 9/DNA
  • wtGFP
  • ZsGreen1

Excitation Intensity Note

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET470/40x (EX) $ 325.00
T495lpxr (BS) $ 225.00
ET525/50m (EM) $ 325.00
$825.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • ET Series
  • White Light

49052

ET - ECFP/EYFP FRET

Excitation Intensity Note
FRET is a challenging fluorescence imaging technique used to demonstrate close proximity between fluorophores. Care must be taken to properly image and quantify numerous controls, especially when using the sensitized emission methodology. See our application note.

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET436/20x (EX) $ 325.00
T455lp (BS) $ 225.00
ET535/30m (EM) $ 325.00
$825.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • ET Series
  • White Light

49011

ET - FITC/Alexa Fluor 488/Fluo3/Oregon Green

Best with these fluorochromes
  • Acridine Orange + DNA
  • Alexa Fluor 488™
  • Atto 488
  • BODIPY FL/pH7.2
  • Calcein
  • Calcium Green™-1
  • Cy2™
  • DyLight 488
  • FAM
  • FITC
  • Fluo-3
  • Fluo-4
  • MitoTracker Green FM/MeOH
  • NBD X/MeOH
  • Oregon Green™ 488
  • SYBR® Green I
  • SYTO 9/DNA

Excitation Intensity Note

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET480/40x (EX) $ 325.00
T510lpxrxt (BS) $ 275.00
ET535/50m (EM) $ 325.00
$825.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • ET Series
  • White Light

49003

ET - EYFP

Best with these fluorochromes
  • Alexa Fluor 514™
  • Atto 488
  • Calcein
  • Calcium Green™-1
  • Citrine
  • EYFP/pH 7
  • FlAsH-CCPFCC
  • Fluo-3
  • mCitrine
  • Oregon Green™ 488
  • Oregon Green™ 514
  • Rhodamine 123
  • Topaz
  • ZsYellow

Excitation Intensity Note

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET500/20x (EX) $ 325.00
T515lp (BS) $ 225.00
ET535/30m (EM) $ 325.00
$825.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • ET Series
  • White Light

49026

ET - Tetracycline

Best with these fluorochromes
  • Lucifer Yellow
  • Tetracycline

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET405/40x (EX) $ 325.00
T470lpxr (BS) $ 225.00
ET550/60m (EM) $ 325.00
$825.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • ET Series
  • White Light

49023

ET - AlexaFluor 514/ZsYellow/Eosin

Best with these fluorochromes
  • Alexa Fluor 514™
  • CAL Fluor® Gold 540
  • CAL Fluor® Orange 560
  • Eosin
  • HEX,SE
  • JOE
  • LysoTracker Yellow HCK-123
  • Rhodamine 6G
  • TET
  • ZsYellow

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET500/40x (EX) $ 325.00
T525lpxr (BS) $ 225.00
ET560/50m (EM) $ 325.00
$825.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • ET Series
  • White Light

49014

ET - mKO/mOrange

Best with these fluorochromes
  • Alexa Fluor 532™
  • CAL Fluor® Gold 540
  • CAL Fluor® Orange 560
  • Cy3™
  • HEX,SE
  • mKO
  • mOrange2
  • Quasar® 570
  • R-phycoerythrin

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET530/30x (EX) $ 325.00
T550lpxr (BS) $ 225.00
ET575/40m (EM) $ 325.00
$825.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • ET Series
  • White Light

49010

ET - R&B Phycoerythrin/mOrange/mKO

Best with these fluorochromes
  • mKO
  • mOrange2
  • R-phycoerythrin

Recommended for use with mercury lamps or metal-halide lamps only.

Excitation Intensity Note

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET546/10x (EX) $ 325.00
T560lpxr (BS) $ 225.00
ET585/40m (EM) $ 325.00
$825.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • ET Series
  • White Light

49016

ET - Cy3/TRITC Longpass

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET545/25x (EX) $ 325.00
T562lpxr (BS) $ 225.00
ET570lp (EM) $ 325.00
$825.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • ET Series
  • White Light

49004

ET - CY3/TRITC

Best with these fluorochromes
  • Alexa Fluor 546™
  • Alexa Fluor 555™
  • Atto 550
  • Cy3™
  • DiI
  • DsRed
  • DyLight 549
  • MitoTracker Orange/MeOH
  • Rhod-2
  • TagRFP
  • TAMRA
  • tdTomato
  • Tetramethylrhodamine isothiocyanate
  • TRITC

Excitation Intensity Note

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET545/25x (EX) $ 325.00
T565lpxr (BS) $ 225.00
ET605/70m (EM) $ 325.00
$825.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • ET Series
  • White Light

49017

ET - mCherry/Texas Red Longpass

Best with these fluorochromes
  • FusionRed
  • mPlum
  • TurboFP650

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET560/40x (EX) $ 325.00
T590lpxr (BS) $ 225.00
ET590lp (EM) $ 325.00
$825.00
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