49000 Series
Chroma’s 49000 series of single band sets features our highest throughput sputter coatings (ET) which achieve the highest transmission levels for interference filters. These filters have very steep transitions from peak transmission to high optical density to best exploit the spectral properties of modern fluorochromes with short Stokes shifts. This is because steep transitions allow for very little spectral separation between excitation and emission bands.
These filters offer the greatest hardness and durability against environmental factors such as high heat and humidity, and are covered by Chroma’s lifetime warranty. All ET filters sets provide zero-pixel-shift image registration* between filter sets, at no additional cost.
*It is important to note that filters are only one factor in achieving perfect image registration in imaging systems. All other components and devices must also be in register.
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49027
ET – Brilliant™ Violet 421, Alexa Fluor 405
This narrow-band filter set is intended for very bright fluorochromes such as BD Horizon Brilliant™ Violet 421. It is sufficiently narrow to allow spectral separation from cyan fluors such as BD Horizon Brilliant™ Violet 480, cyan fluorescent proteins and Aqua FISH probes. This set is also useful for detecting DAPI. DAPI will appear violet instead of blue.
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
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- Transmission
- Optical Density
- Overview
49025
ET - DAPI/FluoroGold Longpass
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
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- Transmission
- Optical Density
- Overview
49053
ET - DAPI for 355-405nm LEDs
Optimized for use with CoolLED light engines.
This set features a wide band excitation filter which is compatible with a broad range of LEDs with peaks between 355-405nm, intended for fluorophores such as DAPI. This set is intended for use with LEDs. If instead, it is used with a broad white light source such as a mercury or metal halide lamp, the excitation filter will transmit very high levels of light to the sample.
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
49000
ET - DAPI
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
49021
ET - EBFP2/Coumarin/Attenuated DAPI
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
49028
ET – DAPI for 395nm light sources
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
49001
ET - ECFP
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
49032
ET – Brilliant™ Violet 480
This narrow-band filter set is intended for very bright fluorochromes such as BD Horizon Brilliant™ Violet 480 (BV 480). When used with BV480, it is sufficiently narrow to allow spectral separation from green fluors such as Alexa fluor 488, FITC, GFP and green FISH probes, as well as separation from violet fluors such as Brilliant Violet™ 421.
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
49013
ET - TFP (Teal FP)
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
49012
ET - FITC/EGFP Longpass
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
49020
ET - Narrow Band EGFP to Minimize Autofluorescence
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
49018
ET - EGFP Longpass
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
49054
ET - GFP for 455-465nm LEDs
Optimized for use with CoolLED light engines.
This set features an excitation filter which is shifted to slightly shorter wavelengths to be most compatible with blue LEDs with peaks between 455-465nm. GFP will be brighter compared with more standard sets that transmit 470-490nm LEDs.
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
49029
ET - EYFP Longpass
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
49002
ET - EGFP (FITC/Cy2)
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
49003
ET - EYFP
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
49052
ET - ECFP/EYFP FRET
Excitation Intensity Note
FRET is a challenging fluorescence imaging technique used to demonstrate close proximity between fluorophores. Care must be taken to properly image and quantify numerous controls, especially when using the sensitized emission methodology. See our application note.
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
49011
ET - FITC/Alexa Fluor 488/Fluo3/Oregon Green
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
49026
ET - Tetracycline
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
49023
ET - AlexaFluor 514/ZsYellow/Eosin
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
49014
ET - mKO/mOrange
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
49010
ET - R&B Phycoerythrin/mOrange/mKO
Recommended for use with mercury lamps or metal-halide lamps only.
Excitation Intensity Note
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
49016
ET - Cy3/TRITC Longpass
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
49004
ET - CY3/TRITC
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
49017
ET - mCherry/Texas Red Longpass
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
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