TIRF
Total Internal Reflection Fluorescence Microscopy (TIRFm) illuminates only the portion of the sample close to the coverslip via the evanescent wave generated when the excitation laser is reflected off the sample/glass interface. The result is a very narrow band of excitation resulting in excellent image contrast. This also means that most of the excitation light is reflected back into the light path of the microscope. The emission filters must attenuate an enormous amount of laser illumination returning through a microscope objective lens, often at extreme angles-of-incidence. In some applications, such as single-molecule TIRF (including PALM and STORM imaging in TIRF mode), the ratio of fluorescence signal-to-illumination could be as low as 1 in 1015:
Three things are necessary to get the signal you need: an emission filter with superior blocking of the excitation light, an ultra-flat dichroic for distortion-free reflection of the excitation laser beam, and no-torque filter cube that can hold that dichroic stress-free.
Our complete TIRF sets remove the guesswork and provide you with the tools to obtain the highest possible signal/noise ratios by blocking the laser illumination at exceedingly high levels. Chroma’s UltraFlat dichroic mirrors mounted in our own metal TIRF cubes provide distortion-free reflection of lasers creating the perfect evanescent wave.
If you don’t see what you are looking for, please contact us. We have a large inventory of filters and a knowledgeable staff ready to help you design and build a filter to meet any specification.
Showing 39 products
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染料
- 10368 (15) Apply 10368 filter
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应用
中心波长/边通波长/陷波波长
- Show:
- Transmission
- Optical Density
TRF49901
ET - 405nm Laser Bandpass Set for TIRF applications
Set includes additional longpass emission filter paired with bandpass emission filter in filter cube for greatly increased attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF89901-EMv2
ET - 405/488/561/640nm Laser Quad Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF89901v2
ET - 405/488/561/640nm Laser Quad Band Set for TIRF applications
Set includes no additional emission filters; instead, the quad band emission filter has increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF69901
ET - 405/488/561nm Laser Triple Band Set for TIRF applications
Set includes no additional emission filters; instead, the triple band emission filter has greatly increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF59901
ET – 405/488nm Laser Dual Band Set for TIRF applications
Set includes no additional emission filters; instead, the dual band emission filter has increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF69902
ET - 405/488/594nm Laser Triple Band Set for TIRF applications
Set includes no additional emission filters; instead, the triple band emission filter has greatly increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF59901-EM
ET – 405/488nm Laser Dual Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF69901-EM
ET - 405/488/561nm Laser Triple Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greater attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF89902
ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications
Set includes no additional emission filters; instead, the quad band emission filter has increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF89902-EM
ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF69902-EM
ET - 405/488/594nm Laser Triple Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greater attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF49902
ET - 440nm Laser Bandpass Set for TIRF applications
Set includes additional longpass emission filter paired with bandpass emission filter in filter cube for greatly increased attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF49903
ET - 440nm Laser Bandpass Set for TIRF applications
Set includes additional longpass emission filter paired with bandpass emission filter in filter cube for greatly increased attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF59902
ET – 442/514nm Laser Dual Band Set for TIRF applications
Set includes no additional emission filters; instead, the dual band emission filter has increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF69904
ET - 442/514/561nm Laser Triple Band Set for TIRF applications
Set includes no additional emission filters; instead, the triple band emission filter has greatly increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF69904-EM
ET - 442/514/561nm Laser Triple Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greater attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF59902-EM
ET – 442/514nm Laser Dual Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF69905
ET - 442/514/594nm Laser Triple Band Set for TIRF applications
Set includes no additional emission filters; instead, the triple band emission filter has greatly increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF69905-EM
ET - 442/514/594nm Laser Triple Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greater attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF59903
ET – 442/532nm Laser Dual Band Set for TIRF applications
Set includes no additional emission filters; instead, the dual band emission filter has increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF59903-EM
ET – 442/532nm Laser Dual Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF49904
ET - 488nm Laser Bandpass Set for TIRF applications
Set includes additional longpass emission filter paired with bandpass emission filter in filter cube for greatly increased attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF59904
ET – 488/561nm Laser Dual Band Set for TIRF applications
Set includes no additional emission filters; instead, the dual band emission filter has increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF59908-EM
ET - PALM Dual Band Emission Set for 488/561nm TIRF applications with 405nm excitation
Set includes the same filter combination as the 59908 PALM set, but with the 2mm TIRF dichroic.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF59904-EM
ET – 488/561nm Laser Dual Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
