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比率测量

Ratiometric imaging uses a ratio of images to determine ion concentration, voltage or pH levels in a cell of a cell network.  One common example is the Fura-2 fluorophore, which absorbs light preferentially at 380nm in high intracellular concentrations of calcium and absorbs light preferentially at 340nm in low concentrations of calcium. By exciting the sample at these two wavelengths and taking a ratio of the image, a semi-quantitative determination of calcium levels is possible.  Other ratiometric fluorophores, like INDO-1,  undergo a change to their emission wavelength under differing ion concentrations. 

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Ratiometric Calcium Indicators - Fluorescence Excitation Spectra of Fura2

 

Ratiometric

 

© 2022 Thermo Fisher Scientific Inc. Used under permission.
www.thermofisher.com

 

 

 

 

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  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • Ratiometric
  • ET Series
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79006

ET - INDO

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET365/10x (EX) $ 325.00
T387lp (BS) $ 275.00
ET405/30m (EM) $ 325.00
ET485/25m (EM) $ 325.00
$1,150.00
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  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • Ratiometric
  • ET Series
  • White Light

79003

ET - Fura 2/TRITC

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET340x (EX) $ 325.00
ET380x (EX) $ 325.00
ET555/25x (EX) $ 325.00
79003bs (BS) $ 400.00
79003m (EM) $ 450.00
$1,750.00
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  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • Ratiometric
  • ET Series
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79001

ET - Fura 2

Excitation Intensity Note

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET340x (EX) $ 325.00
ET380x (EX) $ 325.00
T400lp (BS) $ 225.00
ET510/80m (EM) $ 325.00
$975.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • Ratiometric
  • ET Series
  • White Light

79004

ET - Fura 2/GFP

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET340x (EX) $ 325.00
ET380x (EX) $ 325.00
ET470/40x (EX) $ 325.00
T495lpxru (BS) $ 300.00
ET535/70m (EM) $ 375.00
$1,550.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • Ratiometric
  • ET Series
  • White Light

79005

ET - BCECF

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET436/20x (EX) $ 325.00
ET495/10x (EX) $ 325.00
T510lpxru (BS) $ 300.00
ET540/40m (EM) $ 375.00
$1,250.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • Ratiometric
  • ET Series
  • White Light

79002

ET - Fura 2/BCECF

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET340x (EX) $ 325.00
ET380x (EX) $ 325.00
ET436/20x (EX) $ 325.00
ET495/10x (EX) $ 325.00
T510lpxru (BS) $ 300.00
ET560/80m (EM) $ 375.00
$1,800.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • Ratiometric
  • ET Series
  • White Light

79009

ET - SNARF with 500nm excitation

This filter set is intended for dual-emission ratiometric measurements of the SNARF family of pH indicators when excited with light in the 490-510nm wavelength range.

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET500/20x (EX) $ 325.00
T570lpxr (BS) $ 225.00
ET585/20m (EM) $ 325.00
ET640/20m (EM) $ 325.00
$1,100.00
  • Show:
  • Transmission
  • Optical Density
  • Overview
  • Fluorescence
  • Ratiometric
  • ET Series
  • White Light

79010

ET - SNARF with 550nm excitation

This filter set is intended for dual-emission ratiometric measurements of the SNARF family of pH indicators when excited with light in the 540-560nm wavelength range.

Coating: Sputter/Hard Coated

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.

Listed prices apply to:

  • Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
  Filters in this Set   Price
ET550/20x (EX) $ 325.00
T570lpxr (BS) $ 225.00
ET585/20m (EM) $ 325.00
ET640/20m (EM) $ 325.00
$1,100.00
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