FRET
Förster Resonance Energy Transfer (FRET) is the transfer of photonic energy between two light-sensitive molecules (chromophores). When both chromophores are fluorophores, this is often referred to as Fluorescent Resonance Energy Transfer. The ‘donor’ molecule moves to an excited state after absorbing a photon. The ‘donor’ can then transfer this energy to the ‘acceptor’ chromophore via nonradiative dipole-dipole coupling. This energy transfer can only happen if the donor and acceptor are in close proximity to one another, making this technique very sensitive to small changes in chromophore separation. To image FRET effectively, one must clearly quantify both the donor and the acceptor emission to ensure that any FRET signal does not contain direct fluorescence from either donor or acceptor chromophores.
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Showing 24 products
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染料
- Alexa Fluor 532™ (2) Apply Alexa Fluor 532™ filter
- Alexa Fluor 546™ (11) Apply Alexa Fluor 546™ filter
- Alexa Fluor 555™ (11) Apply Alexa Fluor 555™ filter
- Alexa Fluor 594™ (7) Apply Alexa Fluor 594™ filter
- Alexa Fluor 647™ (6) Apply Alexa Fluor 647™ filter
- Calcium Green™-1 (6) Apply Calcium Green™-1 filter
- Cy3™ (11) Apply Cy3™ filter
- Cy5™ (6) Apply Cy5™ filter
- DiD (6) Apply DiD filter
- DiI (11) Apply DiI filter
- DiO (15) Apply DiO filter
- DsRed (11) Apply DsRed filter
- EGFP (15) Apply EGFP filter
- Eosin (2) Apply Eosin filter
- FM™ 4-64 (6) Apply FM™ 4-64 filter
- Fluo-3 (21) Apply Fluo-3 filter
- Lucifer Yellow (7) Apply Lucifer Yellow filter
- Oregon Green™ 514 (6) Apply Oregon Green™ 514 filter
- Propidium Iodide (13) Apply Propidium Iodide filter
- Resorufin (7) Apply Resorufin filter
- Rhod-2 (11) Apply Rhod-2 filter
- Rhodamine 6G (2) Apply Rhodamine 6G filter
- Rhodamine 123 (6) Apply Rhodamine 123 filter
- Rhodamine Red™-X (18) Apply Rhodamine Red™-X filter
- SYTO® 60 (6) Apply SYTO® 60 filter
- Sulforhodamine 101 (7) Apply Sulforhodamine 101 filter
- TAMRA (11) Apply TAMRA filter
- TO-PRO™-3 (6) Apply TO-PRO™-3 filter
- Texas Red® (7) Apply Texas Red® filter
- Texas Red®-X (7) Apply Texas Red®-X filter
应用
中心波长/边通波长/陷波波长
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TRF59901-EM
ET – 405/488nm Laser Dual Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
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TRF69901-EM
ET - 405/488/561nm Laser Triple Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greater attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
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TRF89902-EM
ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
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TRF69902-EM
ET - 405/488/594nm Laser Triple Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greater attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
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89013
ET - DAPI/FITC/TRITC
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
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89000
ET - Sedat Quad
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
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89014
ET - DAPI/FITC/Texas Red®
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
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89002
ET - ECFP/EYFP
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
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89007
ET - ECFP/EYFP/DsRed
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
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- Transmission
- Optical Density
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89006
ET - ECFP/EYFP/mCherry
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
TRF69904-EM
ET - 442/514/561nm Laser Triple Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greater attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
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- Transmission
- Optical Density
TRF59902-EM
ET – 442/514nm Laser Dual Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
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- Transmission
- Optical Density
TRF69905-EM
ET - 442/514/594nm Laser Triple Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greater attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
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- Transmission
- Optical Density
TRF59903-EM
ET – 442/532nm Laser Dual Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
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- Transmission
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- Overview
89016
ET - FITC/Cy3/Cy5
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
- Overview
89019
ET - FITC/TRITC
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
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- Transmission
- Optical Density
TRF59908-EM
ET - PALM Dual Band Emission Set for 488/561nm TIRF applications with 405nm excitation
Set includes the same filter combination as the 59908 PALM set, but with the 2mm TIRF dichroic.
Coating: Sputter/Hard Coated
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TRF59904-EM
ET – 488/561nm Laser Dual Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
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89021
ET - EGFP/mCherry
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
TRF59905-EM
ET – 488/594nm Laser Dual Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
TRF59906-EM
ET – 488/640nm Laser Dual Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
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- Transmission
- Optical Density
- Overview
89017
ET - FITC/Texas Red®
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
- Show:
- Transmission
- Optical Density
TRF59907-EM
ET – 532/640nm Laser Dual Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
- Show:
- Transmission
- Optical Density
- Overview
89022
ET - Cy3/Cy5
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
