FRET
Förster Resonance Energy Transfer (FRET) is the transfer of photonic energy between two light-sensitive molecules (chromophores). When both chromophores are fluorophores, this is often referred to as Fluorescent Resonance Energy Transfer. The ‘donor’ molecule moves to an excited state after absorbing a photon. The ‘donor’ can then transfer this energy to the ‘acceptor’ chromophore via nonradiative dipole-dipole coupling. This energy transfer can only happen if the donor and acceptor are in close proximity to one another, making this technique very sensitive to small changes in chromophore separation. To image FRET effectively, one must clearly quantify both the donor and the acceptor emission to ensure that any FRET signal does not contain direct fluorescence from either donor or acceptor chromophores.
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- (-) Remove FRET filter FRET
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- (-) Remove 双波段带通 filter 双波段带通
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染料
- Acridine Orange + DNA (1) Apply Acridine Orange + DNA filter
- Alexa Fluor 488™ (1) Apply Alexa Fluor 488™ filter
- Alexa Fluor 532™ (1) Apply Alexa Fluor 532™ filter
- Alexa Fluor 546™ (1) Apply Alexa Fluor 546™ filter
- Alexa Fluor 555™ (1) Apply Alexa Fluor 555™ filter
- Alexa Fluor 568™ (1) Apply Alexa Fluor 568™ filter
- Alexa Fluor 647™ (3) Apply Alexa Fluor 647™ filter
- Allophycocyanin (APC) (3) Apply Allophycocyanin (APC) filter
- AsRed2 (1) Apply AsRed2 filter
- Atto 488 (1) Apply Atto 488 filter
- Atto 550 (1) Apply Atto 550 filter
- Atto 647N (3) Apply Atto 647N filter
- Azami Green (1) Apply Azami Green filter
- BCECF/pH 9.0 (1) Apply BCECF/pH 9.0 filter
- BODIPY FL/pH7.2 (1) Apply BODIPY FL/pH7.2 filter
- CAL Fluor® Gold 540 (1) Apply CAL Fluor® Gold 540 filter
- CAL Fluor® Orange 560 (1) Apply CAL Fluor® Orange 560 filter
- CAL Fluor® Red 590 (1) Apply CAL Fluor® Red 590 filter
- Calcein (1) Apply Calcein filter
- Cy2™ (1) Apply Cy2™ filter
- Cy3.5™ (1) Apply Cy3.5™ filter
- Cy3™ (1) Apply Cy3™ filter
- Cy5™ (3) Apply Cy5™ filter
- DiD (3) Apply DiD filter
- DiI (1) Apply DiI filter
- DiO (1) Apply DiO filter
- Draq5 (3) Apply Draq5 filter
- DsRed (1) Apply DsRed filter
- DyLight 488 (1) Apply DyLight 488 filter
- DyLight 549 (1) Apply DyLight 549 filter
- DyLight 649 (3) Apply DyLight 649 filter
- EGFP (1) Apply EGFP filter
- Emerald GFP (1) Apply Emerald GFP filter
- Eosin (1) Apply Eosin filter
- FAM (1) Apply FAM filter
- FITC (1) Apply FITC filter
- FM™ 1-43 (1) Apply FM™ 1-43 filter
- Fluo-3 (1) Apply Fluo-3 filter
- Fluo-4 (1) Apply Fluo-4 filter
- FusionRed (1) Apply FusionRed filter
- GFP (1) Apply GFP filter
- HEX,SE (1) Apply HEX,SE filter
- LysoTracker Green/pH 5.2 (1) Apply LysoTracker Green/pH 5.2 filter
- LysoTracker Red/pH 5.2 (1) Apply LysoTracker Red/pH 5.2 filter
- LysoTracker Yellow HCK-123 (1) Apply LysoTracker Yellow HCK-123 filter
- MitoTracker Deep Red 633/MeOH (3) Apply MitoTracker Deep Red 633/MeOH filter
- MitoTracker Green FM/MeOH (1) Apply MitoTracker Green FM/MeOH filter
- MitoTracker Orange/MeOH (1) Apply MitoTracker Orange/MeOH filter
- MitoTracker Red/MeOH (1) Apply MitoTracker Red/MeOH filter
- Oregon Green™ 488 (1) Apply Oregon Green™ 488 filter
- Propidium Iodide (2) Apply Propidium Iodide filter
- Quasar® 570 (1) Apply Quasar® 570 filter
- Quasar® 670 (3) Apply Quasar® 670 filter
- R-phycoerythrin (1) Apply R-phycoerythrin filter
- ROX (1) Apply ROX filter
- Rhod-2 (1) Apply Rhod-2 filter
- Rhodamine 6G (1) Apply Rhodamine 6G filter
- Rhodamine Red™-X (1) Apply Rhodamine Red™-X filter
- SYBR® Green I (1) Apply SYBR® Green I filter
- SYTO 9/DNA (1) Apply SYTO 9/DNA filter
- SYTO® 60 (3) Apply SYTO® 60 filter
- TAMRA (1) Apply TAMRA filter
- (-) Remove TO-PRO™-3 filter TO-PRO™-3
- TRITC (1) Apply TRITC filter
- TagRFP (1) Apply TagRFP filter
- Tetramethylrhodamine isothiocyanate (1) Apply Tetramethylrhodamine isothiocyanate filter
- X-rhod-1/Ca2+ (1) Apply X-rhod-1/Ca2+ filter
- ZsGreen1 (1) Apply ZsGreen1 filter
- ZsYellow (1) Apply ZsYellow filter
- mKO (1) Apply mKO filter
- mOrange2 (1) Apply mOrange2 filter
- mWasabi (1) Apply mWasabi filter
- tdTomato (1) Apply tdTomato filter
中心波长/边通波长/陷波波长
TRF59906-EM
ET – 488/640nm Laser Dual Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
为保证足够的平整度,TIRF所用二向色镜为2mm厚
Filters in this Set | Price | ||
---|---|---|---|
ZET488/640x (EX) | $ | 500.00 | |
ZT488/640rpc (BS) | $ | 625.00 | |
ET525/50m (EM) | $ | 365.00 | |
ET655lp (EM) | $ | 365.00 | |
ZET488/640m (EM) | $ | 575.00 |
Mounted in a Laser TIRF for Nikon TE2000/Ti | $2,650.00 | |
Mounted in a Laser TIRF for Olympus BX2 models | $2,775.00 | |
Mounted in a Laser TIRF for Zeiss Axio | $2,525.00 | |
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters | $2,775.00 |
TRF59907-EM
ET – 532/640nm Laser Dual Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
为保证足够的平整度,TIRF所用二向色镜为2mm厚
Filters in this Set | Price | ||
---|---|---|---|
ZET532/640x (EX) | $ | 500.00 | |
ZT532/640rpc (BS) | $ | 625.00 | |
ET585/65m (EM) | $ | 365.00 | |
ET655lp (EM) | $ | 365.00 | |
ZET532/640m (EM) | $ | 575.00 |
Mounted in a Laser TIRF for Nikon TE2000/Ti | $2,650.00 | |
Mounted in a Laser TIRF for Olympus BX2 models | $2,775.00 | |
Mounted in a Laser TIRF for Zeiss Axio | $2,525.00 | |
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters | $2,775.00 |
- Overview
89022
ET - Cy3/Cy5
- Alexa Fluor 546™
- Alexa Fluor 555™
- Alexa Fluor 568™
- Alexa Fluor 647™
- Allophycocyanin (APC)
- AsRed2
- Atto 550
- Atto 647N
- CAL Fluor® Red 590
- Cy3.5™
- Cy3™
- Cy5™
- DiD
- DiI
- Draq5
- DsRed
- DyLight 549
- DyLight 649
- FusionRed
- LysoTracker Red/pH 5.2
- MitoTracker Deep Red 633/MeOH
- MitoTracker Orange/MeOH
- MitoTracker Red/MeOH
- Propidium Iodide
- Quasar® 570
- Quasar® 670
- R-phycoerythrin
- Rhod-2
- Rhodamine Red™-X
- ROX
- SYTO® 60
- TagRFP
- TAMRA
- tdTomato
- Tetramethylrhodamine isothiocyanate
- TO-PRO™-3
- TRITC
- X-rhod-1/Ca2+
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
Filters in this Set | Price | ||
---|---|---|---|
ET545/25x (EX) | $ | 365.00 | |
ET640/30x (EX) | $ | 365.00 | |
59007bs (BS) | $ | 375.00 | |
ET595/50m (EM) | $ | 365.00 | |
ET690/50m (EM) | $ | 365.00 |