FRET & 89000 Series
Förster Resonance Energy Transfer (FRET) is the transfer of photonic energy between two light-sensitive molecules (chromophores). When both chromophores are fluorophores, this is often referred to as Fluorescent Resonance Energy Transfer. The ‘donor’ molecule moves to an excited state after absorbing a photon. The ‘donor’ can then transfer this energy to the ‘acceptor’ chromophore via nonradiative dipole-dipole coupling. This energy transfer can only happen if the donor and acceptor are in close proximity to one another, making this technique very sensitive to small changes in chromophore separation. To image FRET effectively, one must clearly quantify both the donor and the acceptor emission to ensure that any FRET signal does not contain direct fluorescence from either donor or acceptor chromophores.
If you don’t see what you are looking for, please contact us. We have a large inventory of filters and a knowledgeable staff ready to help you design and build a filter to meet any specification.
This series of multi-band filter sets feature our high performance ET sputter coatings. Included are dual, triple and quad-band sets, all of which use single-band excitation and emission filters. These require the use of filter wheels or sliders, or image-splitting devices to hold multiple individual filters. The polychroic beamsplitters remain stationary in a filter cube or similar holder. These sets are image-registered and are particularly useful for co-localization studies, FRET, and simultaneous imaging in live cell applications using image-splitting devices.
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- (-) Remove Texas Red® filter Texas Red®
- (-) Remove 10444 filter 10444
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- (-) Remove Catalog Sets filter Catalog Sets
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- (-) Remove 10444 filter 10444
- (-) Remove Texas Red® filter Texas Red®
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- (-) Remove 89000 Series filter 89000 Series
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