FRET & TRF59900 Series
Förster Resonance Energy Transfer (FRET) is the transfer of photonic energy between two light-sensitive molecules (chromophores). When both chromophores are fluorophores, this is often referred to as Fluorescent Resonance Energy Transfer. The ‘donor’ molecule moves to an excited state after absorbing a photon. The ‘donor’ can then transfer this energy to the ‘acceptor’ chromophore via nonradiative dipole-dipole coupling. This energy transfer can only happen if the donor and acceptor are in close proximity to one another, making this technique very sensitive to small changes in chromophore separation. To image FRET effectively, one must clearly quantify both the donor and the acceptor emission to ensure that any FRET signal does not contain direct fluorescence from either donor or acceptor chromophores.
If you don’t see what you are looking for, please contact us. We have a large inventory of filters and a knowledgeable staff ready to help you design and build a filter to meet any specification.
We offer two versions of each our TRF59900 dual band TIRF filter sets, each providing greater levels of blocking of the TIRF lasers than our 59900 series filter sets. One version (“-EM”) utilizes additional, single band emission filters which match the Dual band emission filter in the filter cube. This option requires an emission filter wheel to house the additional emission filters. The other option relies on one dual band emission filter which provides extra blocking of the TIRF laser, and is housed in the filter cube. The highest signal/noise levels are obtained with the “-EM” versions, however for workers who need very fast switching or who don’t have a filter wheel, the alternate version is an excellent option. The Ultra-Flat Dichroics in our TIRF sets are 2mm thick for distortion-free reflection of TIRF lasers with surface flatness =/> 0.5 waves/inch P-V, RWD. All catalog TIRF sets are mounted in Chroma’s metal TIRF cubes. For more information, please see the supporting materials on our TIRF filter set webpages.
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- (-) Remove FRET filter FRET
- (-) Remove Resorufin filter Resorufin
- (-) Remove TRF59900 Series filter TRF59900 Series
- (-) Remove Catalog Sets filter Catalog Sets
产品类型
- (-) Remove Catalog Sets filter Catalog Sets
染料
- Acridine Orange + DNA (1) Apply Acridine Orange + DNA filter
- Alexa Fluor 488™ (1) Apply Alexa Fluor 488™ filter
- Alexa Fluor 568™ (1) Apply Alexa Fluor 568™ filter
- Alexa Fluor 594™ (1) Apply Alexa Fluor 594™ filter
- AsRed2 (1) Apply AsRed2 filter
- Atto 488 (1) Apply Atto 488 filter
- Azami Green (1) Apply Azami Green filter
- BCECF/pH 9.0 (1) Apply BCECF/pH 9.0 filter
- BODIPY FL/pH7.2 (1) Apply BODIPY FL/pH7.2 filter
- CAL Fluor® Red 590 (1) Apply CAL Fluor® Red 590 filter
- CAL Fluor® Red 610 (1) Apply CAL Fluor® Red 610 filter
- Calcein (1) Apply Calcein filter
- Cy2™ (1) Apply Cy2™ filter
- Cy3.5™ (1) Apply Cy3.5™ filter
- DiO (1) Apply DiO filter
- DyLight 488 (1) Apply DyLight 488 filter
- DyLight 594 (1) Apply DyLight 594 filter
- EGFP (1) Apply EGFP filter
- Emerald GFP (1) Apply Emerald GFP filter
- FAM (1) Apply FAM filter
- FITC (1) Apply FITC filter
- FM™ 1-43 (1) Apply FM™ 1-43 filter
- Fluo-3 (1) Apply Fluo-3 filter
- Fluo-4 (1) Apply Fluo-4 filter
- FusionRed (1) Apply FusionRed filter
- GFP (1) Apply GFP filter
- Killer Red (1) Apply Killer Red filter
- LysoTracker Green/pH 5.2 (1) Apply LysoTracker Green/pH 5.2 filter
- LysoTracker Red/pH 5.2 (1) Apply LysoTracker Red/pH 5.2 filter
- LysoTracker Yellow HCK-123 (1) Apply LysoTracker Yellow HCK-123 filter
- MitoTracker Green FM/MeOH (1) Apply MitoTracker Green FM/MeOH filter
- MitoTracker Red/MeOH (1) Apply MitoTracker Red/MeOH filter
- Oregon Green™ 488 (1) Apply Oregon Green™ 488 filter
- ROX (1) Apply ROX filter
- ReAsH-CCPGCC (1) Apply ReAsH-CCPGCC filter
- (-) Remove Resorufin filter Resorufin
- Rhodamine Red™-X (1) Apply Rhodamine Red™-X filter
- SNARF pH 9.0 (1) Apply SNARF pH 9.0 filter
- SYBR® Green I (1) Apply SYBR® Green I filter
- SYTO 9/DNA (1) Apply SYTO 9/DNA filter
- Sulforhodamine 101 (1) Apply Sulforhodamine 101 filter
- Texas Red® (1) Apply Texas Red® filter
- Texas Red®-X (1) Apply Texas Red®-X filter
- TurboFP650 (1) Apply TurboFP650 filter
- X-rhod-1/Ca2+ (1) Apply X-rhod-1/Ca2+ filter
- ZsGreen1 (1) Apply ZsGreen1 filter
- mCherry (1) Apply mCherry filter
- mKate2 (1) Apply mKate2 filter
- mPlum (1) Apply mPlum filter
- mRFP1 (1) Apply mRFP1 filter
- mWasabi (1) Apply mWasabi filter
产品系列编号
- (-) Remove TRF59900 Series filter TRF59900 Series
中心波长/边通波长/陷波波长
TRF59905-EM
ET – 488/594nm Laser Dual Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
为保证足够的平整度,TIRF所用二向色镜为2mm厚
Filters in this Set | Price | ||
---|---|---|---|
ZET488/594x (EX) | $ | 500.00 | |
ZT488/594rpc (BS) | $ | 625.00 | |
ET525/50m (EM) | $ | 365.00 | |
ET645/75m (EM) | $ | 365.00 | |
ZET488/594m (EM) | $ | 575.00 |
Mounted in a Laser TIRF for Nikon TE2000/Ti | $2,650.00 | |
Mounted in a Laser TIRF for Olympus BX2 models | $2,775.00 | |
Mounted in a Laser TIRF for Zeiss Axio | $2,525.00 | |
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters | $2,775.00 |