FRET & TRF69900 Series
Förster Resonance Energy Transfer (FRET) is the transfer of photonic energy between two light-sensitive molecules (chromophores). When both chromophores are fluorophores, this is often referred to as Fluorescent Resonance Energy Transfer. The ‘donor’ molecule moves to an excited state after absorbing a photon. The ‘donor’ can then transfer this energy to the ‘acceptor’ chromophore via nonradiative dipole-dipole coupling. This energy transfer can only happen if the donor and acceptor are in close proximity to one another, making this technique very sensitive to small changes in chromophore separation. To image FRET effectively, one must clearly quantify both the donor and the acceptor emission to ensure that any FRET signal does not contain direct fluorescence from either donor or acceptor chromophores.
If you don’t see what you are looking for, please contact us. We have a large inventory of filters and a knowledgeable staff ready to help you design and build a filter to meet any specification.
We offer two versions of each our TRF69900 triple band TIRF filter sets, each providing greater levels of blocking of the TIRF lasers than our 69900 series filter sets. One version (“-EM”) utilizes additional, single band emission filters which match the Triple band emission filter in the filter cube. This option requires an emission filter wheel to house the additional emission filters. The other option relies on one triple band emission filter which provides extra blocking of the TIRF laser, and is housed in the filter cube. The highest signal/noise levels are obtained with the “-EM” versions, however for workers who need very fast switching or who don’t have a filter wheel, the alternate version is an excellent option. The Ultra-Flat Dichroics in our TIRF sets are 2mm thick for distortion-free reflection of TIRF lasers with surface flatness =/> 0.5 waves/inch P-V, RWD. All catalog TIRF sets are mounted in Chroma’s metal TIRF cubes. For more information, please see the supporting materials on our TIRF filter set webpages.
Showing 2 products
- (-) Remove FRET filter FRET
- (-) Remove Lucifer Yellow filter Lucifer Yellow
- (-) Remove TRF69900 Series filter TRF69900 Series
- (-) Remove Catalog Sets filter Catalog Sets
产品类型
- (-) Remove Catalog Sets filter Catalog Sets
染料
- Alexa Fluor 514™ (2) Apply Alexa Fluor 514™ filter
- Alexa Fluor 546™ (1) Apply Alexa Fluor 546™ filter
- Alexa Fluor 555™ (1) Apply Alexa Fluor 555™ filter
- Alexa Fluor 568™ (2) Apply Alexa Fluor 568™ filter
- Alexa Fluor 594™ (1) Apply Alexa Fluor 594™ filter
- AsRed2 (2) Apply AsRed2 filter
- Atto 425 (2) Apply Atto 425 filter
- Atto 550 (1) Apply Atto 550 filter
- Brilliant Violet™ 480 (2) Apply Brilliant Violet™ 480 filter
- CAL Fluor® Red 590 (2) Apply CAL Fluor® Red 590 filter
- CAL Fluor® Red 610 (1) Apply CAL Fluor® Red 610 filter
- CFP (2) Apply CFP filter
- Calcium Green™-1 (2) Apply Calcium Green™-1 filter
- Cerulean (2) Apply Cerulean filter
- Citrine (2) Apply Citrine filter
- Cy3.5™ (2) Apply Cy3.5™ filter
- Cy3™ (1) Apply Cy3™ filter
- DiI (1) Apply DiI filter
- DsRed (1) Apply DsRed filter
- DyLight 549 (1) Apply DyLight 549 filter
- DyLight 594 (1) Apply DyLight 594 filter
- ECFP (2) Apply ECFP filter
- EYFP/pH 7 (2) Apply EYFP/pH 7 filter
- FM™ 4-64 (2) Apply FM™ 4-64 filter
- FlAsH-CCPFCC (2) Apply FlAsH-CCPFCC filter
- Fluo-3 (2) Apply Fluo-3 filter
- FusionRed (2) Apply FusionRed filter
- Killer Red (1) Apply Killer Red filter
- (-) Remove Lucifer Yellow filter Lucifer Yellow
- LysoTracker Red/pH 5.2 (2) Apply LysoTracker Red/pH 5.2 filter
- MitoTracker Orange/MeOH (1) Apply MitoTracker Orange/MeOH filter
- MitoTracker Red/MeOH (2) Apply MitoTracker Red/MeOH filter
- Oregon Green™ 514 (2) Apply Oregon Green™ 514 filter
- Propidium Iodide (1) Apply Propidium Iodide filter
- Quasar® 570 (1) Apply Quasar® 570 filter
- R-phycoerythrin (1) Apply R-phycoerythrin filter
- ROX (2) Apply ROX filter
- ReAsH-CCPGCC (1) Apply ReAsH-CCPGCC filter
- Resorufin (1) Apply Resorufin filter
- Rhod-2 (1) Apply Rhod-2 filter
- Rhodamine 123 (2) Apply Rhodamine 123 filter
- Rhodamine Red™-X (2) Apply Rhodamine Red™-X filter
- SNARF pH 9.0 (1) Apply SNARF pH 9.0 filter
- Sulforhodamine 101 (1) Apply Sulforhodamine 101 filter
- TAMRA (1) Apply TAMRA filter
- TRITC (1) Apply TRITC filter
- TagRFP (1) Apply TagRFP filter
- Tetramethylrhodamine isothiocyanate (1) Apply Tetramethylrhodamine isothiocyanate filter
- Texas Red® (1) Apply Texas Red® filter
- Texas Red®-X (1) Apply Texas Red®-X filter
- Topaz (2) Apply Topaz filter
- TurboFP650 (1) Apply TurboFP650 filter
- X-rhod-1/Ca2+ (2) Apply X-rhod-1/Ca2+ filter
- mCherry (1) Apply mCherry filter
- mCitrine (2) Apply mCitrine filter
- mKate2 (1) Apply mKate2 filter
- mKeima Red (2) Apply mKeima Red filter
- mPlum (1) Apply mPlum filter
- mRFP1 (1) Apply mRFP1 filter
- tdTomato (1) Apply tdTomato filter
产品系列编号
- (-) Remove TRF69900 Series filter TRF69900 Series
中心波长/边通波长/陷波波长
TRF69904-EM
ET - 442/514/561nm Laser Triple Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greater attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
为保证足够的平整度,TIRF所用二向色镜为2mm厚
Filters in this Set | Price | ||
---|---|---|---|
ZET442/514/561x (EX) | $ | 550.00 | |
ZT442/514/561rpc (BS) | $ | 650.00 | |
ET480/40m (EM) | $ | 365.00 | |
ET540/30m (EM) | $ | 365.00 | |
ET575lp (EM) | $ | 365.00 | |
ZET442/514/561m (EM) | $ | 575.00 |
Mounted in a Laser TIRF for Nikon TE2000/Ti | $3,100.00 | |
Mounted in a Laser TIRF for Olympus BX2 models | $3,225.00 | |
Mounted in a Laser TIRF for Zeiss Axio | $2,975.00 | |
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters | $3,225.00 |
TRF69905-EM
ET - 442/514/594nm Laser Triple Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greater attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
为保证足够的平整度,TIRF所用二向色镜为2mm厚
Filters in this Set | Price | ||
---|---|---|---|
ZET445/514/594x (EX) | $ | 550.00 | |
ZT445/514/594rpc (BS) | $ | 650.00 | |
ET480/40m (EM) | $ | 365.00 | |
ET550/50m (EM) | $ | 365.00 | |
ET645/75m (EM) | $ | 365.00 | |
ZET445/514/594m (EM) | $ | 575.00 |
Mounted in a Laser TIRF for Nikon TE2000/Ti | $3,100.00 | |
Mounted in a Laser TIRF for Olympus BX2 models | $3,225.00 | |
Mounted in a Laser TIRF for Zeiss Axio | $2,975.00 | |
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters | $3,225.00 |