FRET
Förster Resonance Energy Transfer (FRET) is the transfer of photonic energy between two light-sensitive molecules (chromophores). When both chromophores are fluorophores, this is often referred to as Fluorescent Resonance Energy Transfer. The ‘donor’ molecule moves to an excited state after absorbing a photon. The ‘donor’ can then transfer this energy to the ‘acceptor’ chromophore via nonradiative dipole-dipole coupling. This energy transfer can only happen if the donor and acceptor are in close proximity to one another, making this technique very sensitive to small changes in chromophore separation. To image FRET effectively, one must clearly quantify both the donor and the acceptor emission to ensure that any FRET signal does not contain direct fluorescence from either donor or acceptor chromophores.
If you don’t see what you are looking for, please contact us. We have a large inventory of filters and a knowledgeable staff ready to help you design and build a filter to meet any specification.
Showing 3 products
- (-) Remove FRET filter FRET
- (-) Remove Alexa Fluor 594™ filter Alexa Fluor 594™
- (-) Remove 双波段带通 filter 双波段带通
- (-) Remove Catalog Sets filter Catalog Sets
染料
- Acridine Orange + DNA (3) Apply Acridine Orange + DNA filter
- Alexa Fluor 488™ (3) Apply Alexa Fluor 488™ filter
- Alexa Fluor 568™ (3) Apply Alexa Fluor 568™ filter
- (-) Remove Alexa Fluor 594™ filter Alexa Fluor 594™
- AsRed2 (3) Apply AsRed2 filter
- Atto 488 (3) Apply Atto 488 filter
- Azami Green (3) Apply Azami Green filter
- BCECF/pH 9.0 (3) Apply BCECF/pH 9.0 filter
- BODIPY FL/pH7.2 (3) Apply BODIPY FL/pH7.2 filter
- CAL Fluor® Red 590 (3) Apply CAL Fluor® Red 590 filter
- CAL Fluor® Red 610 (3) Apply CAL Fluor® Red 610 filter
- Calcein (3) Apply Calcein filter
- Cy2™ (3) Apply Cy2™ filter
- Cy3.5™ (3) Apply Cy3.5™ filter
- DiO (3) Apply DiO filter
- DyLight 488 (3) Apply DyLight 488 filter
- DyLight 594 (3) Apply DyLight 594 filter
- EGFP (3) Apply EGFP filter
- Emerald GFP (3) Apply Emerald GFP filter
- FAM (3) Apply FAM filter
- FITC (3) Apply FITC filter
- FM™ 1-43 (3) Apply FM™ 1-43 filter
- Fluo-3 (3) Apply Fluo-3 filter
- Fluo-4 (3) Apply Fluo-4 filter
- FusionRed (3) Apply FusionRed filter
- GFP (3) Apply GFP filter
- Killer Red (3) Apply Killer Red filter
- LysoTracker Green/pH 5.2 (3) Apply LysoTracker Green/pH 5.2 filter
- LysoTracker Red/pH 5.2 (3) Apply LysoTracker Red/pH 5.2 filter
- LysoTracker Yellow HCK-123 (3) Apply LysoTracker Yellow HCK-123 filter
- MitoTracker Green FM/MeOH (3) Apply MitoTracker Green FM/MeOH filter
- MitoTracker Red/MeOH (3) Apply MitoTracker Red/MeOH filter
- Oregon Green™ 488 (3) Apply Oregon Green™ 488 filter
- ROX (3) Apply ROX filter
- ReAsH-CCPGCC (3) Apply ReAsH-CCPGCC filter
- Resorufin (3) Apply Resorufin filter
- Rhodamine Red™-X (3) Apply Rhodamine Red™-X filter
- SNARF pH 9.0 (3) Apply SNARF pH 9.0 filter
- SYBR® Green I (3) Apply SYBR® Green I filter
- SYTO 9/DNA (3) Apply SYTO 9/DNA filter
- Sulforhodamine 101 (3) Apply Sulforhodamine 101 filter
- Texas Red® (3) Apply Texas Red® filter
- Texas Red®-X (3) Apply Texas Red®-X filter
- TurboFP650 (3) Apply TurboFP650 filter
- X-rhod-1/Ca2+ (3) Apply X-rhod-1/Ca2+ filter
- ZsGreen1 (3) Apply ZsGreen1 filter
- mCherry (3) Apply mCherry filter
- mKate2 (3) Apply mKate2 filter
- mPlum (3) Apply mPlum filter
- mRFP1 (3) Apply mRFP1 filter
- mWasabi (3) Apply mWasabi filter
中心波长/边通波长/陷波波长
- Overview
89021
ET - EGFP/mCherry
- Acridine Orange + DNA
- Alexa Fluor 488™
- Alexa Fluor 568™
- Alexa Fluor 594™
- AsRed2
- Atto 488
- Azami Green
- BCECF/pH 9.0
- BODIPY FL/pH7.2
- CAL Fluor® Red 590
- CAL Fluor® Red 610
- Calcein
- Cy2™
- Cy3.5™
- DiO
- DyLight 488
- DyLight 594
- EGFP
- Emerald GFP
- FAM
- FITC
- Fluo-3
- Fluo-4
- FM™ 1-43
- FusionRed
- GFP
- Killer Red
- LysoTracker Green/pH 5.2
- LysoTracker Red/pH 5.2
- LysoTracker Yellow HCK-123
- mCherry
- MitoTracker Green FM/MeOH
- MitoTracker Red/MeOH
- mKate2
- mPlum
- mRFP1
- mWasabi
- Oregon Green™ 488
- ReAsH-CCPGCC
- Resorufin
- Rhodamine Red™-X
- ROX
- SNARF pH 9.0
- Sulforhodamine 101
- SYBR® Green I
- SYTO 9/DNA
- Texas Red®
- Texas Red®-X
- TurboFP650
- X-rhod-1/Ca2+
- ZsGreen1
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
Filters in this Set | Price | ||
---|---|---|---|
ET470/40x (EX) | $ | 365.00 | |
ET572/35x (EX) | $ | 365.00 | |
59022bs (BS) | $ | 375.00 | |
ET525/50m (EM) | $ | 365.00 | |
ET632/60m (EM) | $ | 365.00 |
TRF59905-EM
ET – 488/594nm Laser Dual Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
为保证足够的平整度,TIRF所用二向色镜为2mm厚
Filters in this Set | Price | ||
---|---|---|---|
ZET488/594x (EX) | $ | 500.00 | |
ZT488/594rpc (BS) | $ | 625.00 | |
ET525/50m (EM) | $ | 365.00 | |
ET645/75m (EM) | $ | 365.00 | |
ZET488/594m (EM) | $ | 575.00 |
Mounted in a Laser TIRF for Nikon TE2000/Ti | $2,650.00 | |
Mounted in a Laser TIRF for Olympus BX2 models | $2,775.00 | |
Mounted in a Laser TIRF for Zeiss Axio | $2,525.00 | |
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters | $2,775.00 |
- Overview
89017
ET - FITC/Texas Red®
- Acridine Orange + DNA
- Alexa Fluor 488™
- Alexa Fluor 568™
- Alexa Fluor 594™
- AsRed2
- Atto 488
- Azami Green
- BCECF/pH 9.0
- BODIPY FL/pH7.2
- CAL Fluor® Red 590
- CAL Fluor® Red 610
- Calcein
- Cy2™
- Cy3.5™
- DiO
- DyLight 488
- DyLight 594
- EGFP
- Emerald GFP
- FAM
- FITC
- Fluo-3
- Fluo-4
- FM™ 1-43
- FusionRed
- GFP
- Killer Red
- LysoTracker Green/pH 5.2
- LysoTracker Red/pH 5.2
- LysoTracker Yellow HCK-123
- mCherry
- MitoTracker Green FM/MeOH
- MitoTracker Red/MeOH
- mKate2
- mPlum
- mRFP1
- mWasabi
- Oregon Green™ 488
- ReAsH-CCPGCC
- Resorufin
- Rhodamine Red™-X
- ROX
- SNARF pH 9.0
- Sulforhodamine 101
- SYBR® Green I
- SYTO 9/DNA
- Texas Red®
- Texas Red®-X
- TurboFP650
- X-rhod-1/Ca2+
- ZsGreen1
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
Filters in this Set | Price | ||
---|---|---|---|
ET490/20x (EX) | $ | 365.00 | |
ET572/35x (EX) | $ | 365.00 | |
69002bs (BS) | $ | 425.00 | |
ET535/50m (EM) | $ | 365.00 | |
ET632/60m (EM) | $ | 365.00 |