FRET
Förster Resonance Energy Transfer (FRET) is the transfer of photonic energy between two light-sensitive molecules (chromophores). When both chromophores are fluorophores, this is often referred to as Fluorescent Resonance Energy Transfer. The ‘donor’ molecule moves to an excited state after absorbing a photon. The ‘donor’ can then transfer this energy to the ‘acceptor’ chromophore via nonradiative dipole-dipole coupling. This energy transfer can only happen if the donor and acceptor are in close proximity to one another, making this technique very sensitive to small changes in chromophore separation. To image FRET effectively, one must clearly quantify both the donor and the acceptor emission to ensure that any FRET signal does not contain direct fluorescence from either donor or acceptor chromophores.
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产品类型
- (-) Remove Catalog Sets filter Catalog Sets
染料
- Alexa Fluor 514™ (2) Apply Alexa Fluor 514™ filter
- Alexa Fluor 546™ (2) Apply Alexa Fluor 546™ filter
- Alexa Fluor 555™ (2) Apply Alexa Fluor 555™ filter
- Alexa Fluor 568™ (2) Apply Alexa Fluor 568™ filter
- AsRed2 (2) Apply AsRed2 filter
- Atto 425 (2) Apply Atto 425 filter
- Atto 550 (2) Apply Atto 550 filter
- Brilliant Violet™ 480 (2) Apply Brilliant Violet™ 480 filter
- CAL Fluor® Red 590 (2) Apply CAL Fluor® Red 590 filter
- CFP (2) Apply CFP filter
- Calcium Green™-1 (2) Apply Calcium Green™-1 filter
- Cerulean (2) Apply Cerulean filter
- Citrine (2) Apply Citrine filter
- Cy3.5™ (2) Apply Cy3.5™ filter
- Cy3™ (2) Apply Cy3™ filter
- DiI (2) Apply DiI filter
- DsRed (2) Apply DsRed filter
- DyLight 549 (2) Apply DyLight 549 filter
- ECFP (2) Apply ECFP filter
- EYFP/pH 7 (2) Apply EYFP/pH 7 filter
- FM™ 4-64 (2) Apply FM™ 4-64 filter
- FlAsH-CCPFCC (2) Apply FlAsH-CCPFCC filter
- Fluo-3 (2) Apply Fluo-3 filter
- FusionRed (2) Apply FusionRed filter
- (-) Remove Lucifer Yellow filter Lucifer Yellow
- LysoTracker Red/pH 5.2 (2) Apply LysoTracker Red/pH 5.2 filter
- MitoTracker Orange/MeOH (2) Apply MitoTracker Orange/MeOH filter
- MitoTracker Red/MeOH (2) Apply MitoTracker Red/MeOH filter
- Oregon Green™ 514 (2) Apply Oregon Green™ 514 filter
- Propidium Iodide (2) Apply Propidium Iodide filter
- Quasar® 570 (2) Apply Quasar® 570 filter
- R-phycoerythrin (2) Apply R-phycoerythrin filter
- ROX (2) Apply ROX filter
- Rhod-2 (2) Apply Rhod-2 filter
- Rhodamine 123 (2) Apply Rhodamine 123 filter
- Rhodamine Red™-X (2) Apply Rhodamine Red™-X filter
- (-) Remove TAMRA filter TAMRA
- TRITC (2) Apply TRITC filter
- TagRFP (2) Apply TagRFP filter
- Tetramethylrhodamine isothiocyanate (2) Apply Tetramethylrhodamine isothiocyanate filter
- Topaz (2) Apply Topaz filter
- X-rhod-1/Ca2+ (2) Apply X-rhod-1/Ca2+ filter
- mCitrine (2) Apply mCitrine filter
- mKeima Red (2) Apply mKeima Red filter
- tdTomato (2) Apply tdTomato filter
中心波长/边通波长/陷波波长
- Overview
89007
ET - ECFP/EYFP/DsRed
- Alexa Fluor 514™
- Alexa Fluor 546™
- Alexa Fluor 555™
- Alexa Fluor 568™
- AsRed2
- Atto 425
- Atto 550
- Brilliant Violet™ 480
- CAL Fluor® Red 590
- Calcium Green™-1
- Cerulean
- CFP
- Citrine
- Cy3.5™
- Cy3™
- DiI
- DsRed
- DyLight 549
- ECFP
- EYFP/pH 7
- FlAsH-CCPFCC
- Fluo-3
- FM™ 4-64
- FusionRed
- Lucifer Yellow
- LysoTracker Red/pH 5.2
- mCitrine
- MitoTracker Orange/MeOH
- MitoTracker Red/MeOH
- mKeima Red
- Oregon Green™ 514
- Propidium Iodide
- Quasar® 570
- R-phycoerythrin
- Rhod-2
- Rhodamine 123
- Rhodamine Red™-X
- ROX
- TagRFP
- TAMRA
- tdTomato
- Tetramethylrhodamine isothiocyanate
- Topaz
- TRITC
- X-rhod-1/Ca2+
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
Filters in this Set | Price | ||
---|---|---|---|
ET430/24x (EX) | $ | 365.00 | |
ET500/20x (EX) | $ | 365.00 | |
ET555/20x (EX) | $ | 365.00 | |
89007bs (BS) | $ | 425.00 | |
ET470/24m (EM) | $ | 365.00 | |
ET535/30m (EM) | $ | 365.00 | |
ET600/50m (EM) | $ | 365.00 |
TRF69904-EM
ET - 442/514/561nm Laser Triple Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greater attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
为保证足够的平整度,TIRF所用二向色镜为2mm厚
Filters in this Set | Price | ||
---|---|---|---|
ZET442/514/561x (EX) | $ | 550.00 | |
ZT442/514/561rpc (BS) | $ | 650.00 | |
ET480/40m (EM) | $ | 365.00 | |
ET540/30m (EM) | $ | 365.00 | |
ET575lp (EM) | $ | 365.00 | |
ZET442/514/561m (EM) | $ | 575.00 |
Mounted in a Laser TIRF for Nikon TE2000/Ti | $3,100.00 | |
Mounted in a Laser TIRF for Olympus BX2 models | $3,225.00 | |
Mounted in a Laser TIRF for Zeiss Axio | $2,975.00 | |
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters | $3,225.00 |