TIRF
Total Internal Reflection Fluorescence Microscopy (TIRFm) illuminates only the portion of the sample close to the coverslip via the evanescent wave generated when the excitation laser is reflected off the sample/glass interface. The result is a very narrow band of excitation resulting in excellent image contrast. This also means that most of the excitation light is reflected back into the light path of the microscope. The emission filters must attenuate an enormous amount of laser illumination returning through a microscope objective lens, often at extreme angles-of-incidence. In some applications, such as single-molecule TIRF (including PALM and STORM imaging in TIRF mode), the ratio of fluorescence signal-to-illumination could be as low as 1 in 1015:
Three things are necessary to get the signal you need: an emission filter with superior blocking of the excitation light, an ultra-flat dichroic for distortion-free reflection of the excitation laser beam, and no-torque filter cube that can hold that dichroic stress-free.
Our complete TIRF sets remove the guesswork and provide you with the tools to obtain the highest possible signal/noise ratios by blocking the laser illumination at exceedingly high levels. Chroma’s UltraFlat dichroic mirrors mounted in our own metal TIRF cubes provide distortion-free reflection of lasers creating the perfect evanescent wave.
If you don’t see what you are looking for, please contact us. We have a large inventory of filters and a knowledgeable staff ready to help you design and build a filter to meet any specification.
Showing 7 products
- (-) Remove TIRF filter TIRF
- (-) Remove Reclaimed Cubes filter Reclaimed Cubes
产品类型
染料
- Acridine Orange + DNA (1) Apply Acridine Orange + DNA filter
- Alexa Fluor 488™ (1) Apply Alexa Fluor 488™ filter
- Alexa Fluor 514™ (1) Apply Alexa Fluor 514™ filter
- Alexa Fluor 532™ (2) Apply Alexa Fluor 532™ filter
- Alexa Fluor 546™ (1) Apply Alexa Fluor 546™ filter
- Alexa Fluor 555™ (1) Apply Alexa Fluor 555™ filter
- Alexa Fluor 568™ (1) Apply Alexa Fluor 568™ filter
- Alexa Fluor 647™ (2) Apply Alexa Fluor 647™ filter
- Allophycocyanin (APC) (2) Apply Allophycocyanin (APC) filter
- AsRed2 (1) Apply AsRed2 filter
- Atto 425 (1) Apply Atto 425 filter
- Atto 488 (1) Apply Atto 488 filter
- Atto 550 (1) Apply Atto 550 filter
- Atto 647N (2) Apply Atto 647N filter
- Azami Green (1) Apply Azami Green filter
- BCECF/pH 9.0 (1) Apply BCECF/pH 9.0 filter
- BODIPY FL/pH7.2 (1) Apply BODIPY FL/pH7.2 filter
- Brilliant Violet™ 480 (1) Apply Brilliant Violet™ 480 filter
- CAL Fluor® Gold 540 (2) Apply CAL Fluor® Gold 540 filter
- CAL Fluor® Orange 560 (2) Apply CAL Fluor® Orange 560 filter
- CAL Fluor® Red 590 (1) Apply CAL Fluor® Red 590 filter
- CFP (1) Apply CFP filter
- Calcein (1) Apply Calcein filter
- Calcium Green™-1 (1) Apply Calcium Green™-1 filter
- Cerulean (1) Apply Cerulean filter
- Citrine (1) Apply Citrine filter
- Cy2™ (1) Apply Cy2™ filter
- Cy3.5™ (1) Apply Cy3.5™ filter
- Cy3™ (1) Apply Cy3™ filter
- Cy5™ (2) Apply Cy5™ filter
- DiD (2) Apply DiD filter
- DiI (1) Apply DiI filter
- DiO (1) Apply DiO filter
- Draq5 (2) Apply Draq5 filter
- DsRed (1) Apply DsRed filter
- DyLight 488 (1) Apply DyLight 488 filter
- DyLight 549 (1) Apply DyLight 549 filter
- DyLight 649 (2) Apply DyLight 649 filter
- ECFP (1) Apply ECFP filter
- EGFP (1) Apply EGFP filter
- EYFP/pH 7 (1) Apply EYFP/pH 7 filter
- Emerald GFP (1) Apply Emerald GFP filter
- Eosin (2) Apply Eosin filter
- FAM (1) Apply FAM filter
- FITC (1) Apply FITC filter
- FM™ 1-43 (1) Apply FM™ 1-43 filter
- FM™ 4-64 (1) Apply FM™ 4-64 filter
- FlAsH-CCPFCC (1) Apply FlAsH-CCPFCC filter
- Fluo-3 (2) Apply Fluo-3 filter
- Fluo-4 (1) Apply Fluo-4 filter
- FusionRed (1) Apply FusionRed filter
- GFP (1) Apply GFP filter
- HEX,SE (2) Apply HEX,SE filter
- Lucifer Yellow (1) Apply Lucifer Yellow filter
- LysoTracker Green/pH 5.2 (1) Apply LysoTracker Green/pH 5.2 filter
- LysoTracker Red/pH 5.2 (1) Apply LysoTracker Red/pH 5.2 filter
- LysoTracker Yellow HCK-123 (1) Apply LysoTracker Yellow HCK-123 filter
- MitoTracker Deep Red 633/MeOH (2) Apply MitoTracker Deep Red 633/MeOH filter
- MitoTracker Green FM/MeOH (1) Apply MitoTracker Green FM/MeOH filter
- MitoTracker Orange/MeOH (1) Apply MitoTracker Orange/MeOH filter
- MitoTracker Red/MeOH (1) Apply MitoTracker Red/MeOH filter
- Oregon Green™ 488 (1) Apply Oregon Green™ 488 filter
- Oregon Green™ 514 (1) Apply Oregon Green™ 514 filter
- Propidium Iodide (3) Apply Propidium Iodide filter
- Quasar® 570 (1) Apply Quasar® 570 filter
- Quasar® 670 (2) Apply Quasar® 670 filter
- R-phycoerythrin (1) Apply R-phycoerythrin filter
- ROX (1) Apply ROX filter
- Rhod-2 (1) Apply Rhod-2 filter
- Rhodamine 6G (2) Apply Rhodamine 6G filter
- Rhodamine 123 (1) Apply Rhodamine 123 filter
- Rhodamine Red™-X (1) Apply Rhodamine Red™-X filter
- SYBR® Green I (1) Apply SYBR® Green I filter
- SYTO 9/DNA (1) Apply SYTO 9/DNA filter
- SYTO® 60 (2) Apply SYTO® 60 filter
- TAMRA (1) Apply TAMRA filter
- TO-PRO™-3 (2) Apply TO-PRO™-3 filter
- TRITC (1) Apply TRITC filter
- TagRFP (1) Apply TagRFP filter
- Tetramethylrhodamine isothiocyanate (1) Apply Tetramethylrhodamine isothiocyanate filter
- Topaz (1) Apply Topaz filter
- X-rhod-1/Ca2+ (1) Apply X-rhod-1/Ca2+ filter
- ZsGreen1 (1) Apply ZsGreen1 filter
- ZsYellow (2) Apply ZsYellow filter
- mCitrine (1) Apply mCitrine filter
- mKO (2) Apply mKO filter
- mKeima Red (1) Apply mKeima Red filter
- mOrange2 (2) Apply mOrange2 filter
- mWasabi (1) Apply mWasabi filter
- tdTomato (1) Apply tdTomato filter
中心波长/边通波长/陷波波长
TRF49901
NC479380 - ET - 405nm Laser Bandpass Set for TIRF applications
Premounted in a Laser TIRF for Olympus BX2 models (91041)
Qty in Stock: 1
Set includes additional longpass emission filter paired with bandpass emission filter in filter cube for greatly increased attenuation of TIRF lasers.
Filters in this Set | Price | ||
---|---|---|---|
ZET405/20x (EX) | $ | 375.00 | |
ZT405rdc (BS) | $ | 350.00 | |
ET425lp (EM) | $ | 365.00 | |
ET460/50m (EM) | $ | 365.00 | |
New Set Price | $ | 1,400.00 | |
New Cube Price | $ | 675.00 | |
New Set/Cube Cost | $ | 676.00 | |
Reclaimed Cube Cost | $ | 1,122.50 |
TRF59902-EM
NC675449 - ET – 442/514nm Laser Dual Band Set for TIRF applications
Premounted in a Laser TIRF for Olympus BX2 models (91041)
Qty in Stock: 1
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Filters in this Set | Price | ||
---|---|---|---|
ZET442/514x (EX) | $ | 500.00 | |
ZT442/514rpc (BS) | $ | 525.00 | |
ET480/40m (EM) | $ | 365.00 | |
ET545/40m (EM) | $ | 365.00 | |
ZET442/514m (EM) | $ | 575.00 | |
New Set Price | $ | 2,100.00 | |
New Cube Price | $ | 675.00 | |
New Set/Cube Cost | $ | 677.00 | |
Reclaimed Cube Cost | $ | 1,472.50 |
TRF49904
NC926036 - ET - 488nm Laser Bandpass Set for TIRF applications
Premounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters (91044)
Qty in Stock: 1
Set includes additional longpass emission filter paired with bandpass emission filter in filter cube for greatly increased attenuation of TIRF lasers.
Filters in this Set | Price | ||
---|---|---|---|
ZET488/10x (EX) | $ | 375.00 | |
ZT488rdc (BS) | $ | 350.00 | |
ET500lp (EM) | $ | 365.00 | |
ET525/50m (EM) | $ | 365.00 | |
New Set Price | $ | 1,400.00 | |
New Cube Price | $ | 675.00 | |
New Set/Cube Cost | $ | 676.00 | |
Reclaimed Cube Cost | $ | 1,122.50 |
TRF59904
NC901795 - ET – 488/561nm Laser Dual Band Set for TIRF applications
Premounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters (91044)
Qty in Stock: 1
Set includes no additional emission filters; instead, the dual band emission filter has increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Filters in this Set | Price | ||
---|---|---|---|
ZET488/561x (EX) | $ | 500.00 | |
ZT488/561rpc (BS) | $ | 525.00 | |
ZET488/561m-TRF (EM) | $ | 875.00 | |
New Set Price | $ | 1,800.00 | |
New Cube Price | $ | 675.00 | |
New Set/Cube Cost | $ | 676.00 | |
Reclaimed Cube Cost | $ | 1,322.50 |
TRF59907
NC806191 - ET – 532/640nm Laser Dual Band Set for TIRF applications
Premounted in a Laser TIRF for Nikon TE2000/Ti (91032)
Qty in Stock: 1
Set includes no additional emission filters; instead, the dual band emission filter has increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Filters in this Set | Price | ||
---|---|---|---|
ZET532/640x (EX) | $ | 500.00 | |
ZT532/640rpc (BS) | $ | 525.00 | |
ZET532/640m-TRF (EM) | $ | 875.00 | |
New Set Price | $ | 1,800.00 | |
New Cube Price | $ | 550.00 | |
New Set/Cube Cost | $ | 551.00 | |
Reclaimed Cube Cost | $ | 1,235.00 |
TRF59907
NC892950 - ET – 532/640nm Laser Dual Band Set for TIRF applications
Premounted in a Laser TIRF for Olympus BX2 models (91041)
Qty in Stock: 1
Set includes no additional emission filters; instead, the dual band emission filter has increased blocking for greater attenuation of TIRF lasers without the use of additional filters.
Filters in this Set | Price | ||
---|---|---|---|
ZET532/640x (EX) | $ | 500.00 | |
ZT532/640rpc (BS) | $ | 525.00 | |
ZET532/640m-TRF (EM) | $ | 875.00 | |
New Set Price | $ | 1,800.00 | |
New Cube Price | $ | 675.00 | |
New Set/Cube Cost | $ | 676.00 | |
Reclaimed Cube Cost | $ | 1,322.50 |
TRF49913
NC939040 - ET - 633-640nm Laser Longpass Set for TIRF applications
Premounted in a Laser TIRF for Olympus BX2 models (91041)
Qty in Stock: 1
This set uses a longpass emission filter with greatly increased blocking for greater attenuation of TIRF lasers without the use of an additional emission filter.
Filters in this Set | Price | ||
---|---|---|---|
ZET635/20x (EX) | $ | 375.00 | |
ZT640rdc (BS) | $ | 350.00 | |
ET655lp-TRF (EM) | $ | 575.00 | |
New Set Price | $ | 1,300.00 | |
New Cube Price | $ | 675.00 | |
New Set/Cube Cost | $ | 676.00 | |
Reclaimed Cube Cost | $ | 1,122.50 |