FRET
Förster Resonance Energy Transfer (FRET) is the transfer of photonic energy between two light-sensitive molecules (chromophores). When both chromophores are fluorophores, this is often referred to as Fluorescent Resonance Energy Transfer. The ‘donor’ molecule moves to an excited state after absorbing a photon. The ‘donor’ can then transfer this energy to the ‘acceptor’ chromophore via nonradiative dipole-dipole coupling. This energy transfer can only happen if the donor and acceptor are in close proximity to one another, making this technique very sensitive to small changes in chromophore separation. To image FRET effectively, one must clearly quantify both the donor and the acceptor emission to ensure that any FRET signal does not contain direct fluorescence from either donor or acceptor chromophores.
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染料
- Acridine Orange + DNA (9) Apply Acridine Orange + DNA filter
- Alexa Fluor 350™ (7) Apply Alexa Fluor 350™ filter
- Alexa Fluor 405 (7) Apply Alexa Fluor 405 filter
- Alexa Fluor 488™ (9) Apply Alexa Fluor 488™ filter
- Alexa Fluor 532™ (1) Apply Alexa Fluor 532™ filter
- Alexa Fluor 546™ (10) Apply Alexa Fluor 546™ filter
- Alexa Fluor 555™ (10) Apply Alexa Fluor 555™ filter
- Alexa Fluor 568™ (10) Apply Alexa Fluor 568™ filter
- Alexa Fluor 647™ (12) Apply Alexa Fluor 647™ filter
- Allophycocyanin (APC) (12) Apply Allophycocyanin (APC) filter
- AsRed2 (10) Apply AsRed2 filter
- Atto 488 (9) Apply Atto 488 filter
- Atto 550 (10) Apply Atto 550 filter
- Atto 647N (12) Apply Atto 647N filter
- Azami Green (9) Apply Azami Green filter
- BCECF/pH 9.0 (9) Apply BCECF/pH 9.0 filter
- BODIPY FL/pH7.2 (9) Apply BODIPY FL/pH7.2 filter
- Biosearch Blue (7) Apply Biosearch Blue filter
- Brilliant Violet™ 421 (7) Apply Brilliant Violet™ 421 filter
- CAL Fluor® Gold 540 (1) Apply CAL Fluor® Gold 540 filter
- CAL Fluor® Orange 560 (1) Apply CAL Fluor® Orange 560 filter
- CAL Fluor® Red 590 (10) Apply CAL Fluor® Red 590 filter
- Calcein (9) Apply Calcein filter
- Cy2™ (9) Apply Cy2™ filter
- Cy3.5™ (10) Apply Cy3.5™ filter
- Cy3™ (10) Apply Cy3™ filter
- Cy5™ (12) Apply Cy5™ filter
- DAPI (7) Apply DAPI filter
- (-) Remove DiD filter DiD
- DiI (10) Apply DiI filter
- DiO (9) Apply DiO filter
- Draq5 (12) Apply Draq5 filter
- DsRed (10) Apply DsRed filter
- DyLight 350 (7) Apply DyLight 350 filter
- DyLight 405 (7) Apply DyLight 405 filter
- DyLight 488 (9) Apply DyLight 488 filter
- DyLight 549 (10) Apply DyLight 549 filter
- DyLight 649 (12) Apply DyLight 649 filter
- EBFP2 (7) Apply EBFP2 filter
- EGFP (9) Apply EGFP filter
- Emerald GFP (9) Apply Emerald GFP filter
- Eosin (1) Apply Eosin filter
- FAM (9) Apply FAM filter
- FITC (9) Apply FITC filter
- FM™ 1-43 (9) Apply FM™ 1-43 filter
- Fluo-3 (9) Apply Fluo-3 filter
- Fluo-4 (9) Apply Fluo-4 filter
- FusionRed (10) Apply FusionRed filter
- GFP (9) Apply GFP filter
- HEX,SE (1) Apply HEX,SE filter
- Hoechst 33258 (7) Apply Hoechst 33258 filter
- LysoTracker Blue/MeOH (7) Apply LysoTracker Blue/MeOH filter
- LysoTracker Green/pH 5.2 (9) Apply LysoTracker Green/pH 5.2 filter
- LysoTracker Red/pH 5.2 (10) Apply LysoTracker Red/pH 5.2 filter
- LysoTracker Yellow HCK-123 (9) Apply LysoTracker Yellow HCK-123 filter
- MitoTracker Deep Red 633/MeOH (12) Apply MitoTracker Deep Red 633/MeOH filter
- MitoTracker Green FM/MeOH (9) Apply MitoTracker Green FM/MeOH filter
- MitoTracker Orange/MeOH (10) Apply MitoTracker Orange/MeOH filter
- MitoTracker Red/MeOH (10) Apply MitoTracker Red/MeOH filter
- Oregon Green™ 488 (9) Apply Oregon Green™ 488 filter
- Pacific Blue (7) Apply Pacific Blue filter
- Propidium Iodide (11) Apply Propidium Iodide filter
- Qdot 525 (7) Apply Qdot 525 filter
- Qdot 585 (7) Apply Qdot 585 filter
- Qdot 605 (7) Apply Qdot 605 filter
- Qdot 705 (7) Apply Qdot 705 filter
- Quasar® 570 (10) Apply Quasar® 570 filter
- Quasar® 670 (12) Apply Quasar® 670 filter
- R-phycoerythrin (10) Apply R-phycoerythrin filter
- ROX (10) Apply ROX filter
- Rhod-2 (10) Apply Rhod-2 filter
- Rhodamine 6G (1) Apply Rhodamine 6G filter
- Rhodamine Red™-X (10) Apply Rhodamine Red™-X filter
- SYBR® Green I (9) Apply SYBR® Green I filter
- SYTO 9/DNA (9) Apply SYTO 9/DNA filter
- SYTO® 60 (12) Apply SYTO® 60 filter
- T-Sapphire (7) Apply T-Sapphire filter
- TAMRA (10) Apply TAMRA filter
- TO-PRO™-3 (12) Apply TO-PRO™-3 filter
- TRITC (10) Apply TRITC filter
- TagBFP (7) Apply TagBFP filter
- TagRFP (10) Apply TagRFP filter
- Tetramethylrhodamine isothiocyanate (10) Apply Tetramethylrhodamine isothiocyanate filter
- X-rhod-1/Ca2+ (10) Apply X-rhod-1/Ca2+ filter
- ZsGreen1 (9) Apply ZsGreen1 filter
- ZsYellow (1) Apply ZsYellow filter
- mKO (1) Apply mKO filter
- mOrange2 (1) Apply mOrange2 filter
- mWasabi (9) Apply mWasabi filter
- tdTomato (10) Apply tdTomato filter
中心波长/边通波长/陷波波长
- Overview
89022
ET - Cy3/Cy5
- Alexa Fluor 546™
- Alexa Fluor 555™
- Alexa Fluor 568™
- Alexa Fluor 647™
- Allophycocyanin (APC)
- AsRed2
- Atto 550
- Atto 647N
- CAL Fluor® Red 590
- Cy3.5™
- Cy3™
- Cy5™
- DiD
- DiI
- Draq5
- DsRed
- DyLight 549
- DyLight 649
- FusionRed
- LysoTracker Red/pH 5.2
- MitoTracker Deep Red 633/MeOH
- MitoTracker Orange/MeOH
- MitoTracker Red/MeOH
- Propidium Iodide
- Quasar® 570
- Quasar® 670
- R-phycoerythrin
- Rhod-2
- Rhodamine Red™-X
- ROX
- SYTO® 60
- TagRFP
- TAMRA
- tdTomato
- Tetramethylrhodamine isothiocyanate
- TO-PRO™-3
- TRITC
- X-rhod-1/Ca2+
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
Filters in this Set | Price | ||
---|---|---|---|
ET545/25x (EX) | $ | 365.00 | |
ET640/30x (EX) | $ | 365.00 | |
59007bs (BS) | $ | 375.00 | |
ET595/50m (EM) | $ | 365.00 | |
ET690/50m (EM) | $ | 365.00 |
89022
NC788100 - ET - Cy3/Cy5
Premounted in a Nikon TE2000/Ti (91020)
Qty in Stock: 1
Filters in this Set | Price | ||
---|---|---|---|
ET545/25x (EX) | $ | 365.00 | |
ET640/30x (EX) | $ | 365.00 | |
59007bs (BS) | $ | 375.00 | |
ET595/50m (EM) | $ | 365.00 | |
ET690/50m (EM) | $ | 365.00 | |
New Set Price | $ | 1,550.00 | |
New Cube Price | $ | 490.00 | |
New Set/Cube Cost | $ | 491.00 | |
Reclaimed Cube Cost | $ | 1,028.60 |
TRF59907-EM
ET – 532/640nm Laser Dual Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
为保证足够的平整度,TIRF所用二向色镜为2mm厚
Filters in this Set | Price | ||
---|---|---|---|
ZET532/640x (EX) | $ | 500.00 | |
ZT532/640rpc (BS) | $ | 625.00 | |
ET585/65m (EM) | $ | 365.00 | |
ET655lp (EM) | $ | 365.00 | |
ZET532/640m (EM) | $ | 575.00 |
Mounted in a Laser TIRF for Nikon TE2000/Ti | $2,650.00 | |
Mounted in a Laser TIRF for Olympus BX2 models | $2,775.00 | |
Mounted in a Laser TIRF for Zeiss Axio | $2,525.00 | |
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters | $2,775.00 |
TRF59906-EM
ET – 488/640nm Laser Dual Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
为保证足够的平整度,TIRF所用二向色镜为2mm厚
Filters in this Set | Price | ||
---|---|---|---|
ZET488/640x (EX) | $ | 500.00 | |
ZT488/640rpc (BS) | $ | 625.00 | |
ET525/50m (EM) | $ | 365.00 | |
ET655lp (EM) | $ | 365.00 | |
ZET488/640m (EM) | $ | 575.00 |
Mounted in a Laser TIRF for Nikon TE2000/Ti | $2,650.00 | |
Mounted in a Laser TIRF for Olympus BX2 models | $2,775.00 | |
Mounted in a Laser TIRF for Zeiss Axio | $2,525.00 | |
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters | $2,775.00 |
- Overview
89016
ET - FITC/Cy3/Cy5
- Acridine Orange + DNA
- Alexa Fluor 488™
- Alexa Fluor 546™
- Alexa Fluor 555™
- Alexa Fluor 568™
- Alexa Fluor 647™
- Allophycocyanin (APC)
- AsRed2
- Atto 488
- Atto 550
- Atto 647N
- Azami Green
- BCECF/pH 9.0
- BODIPY FL/pH7.2
- CAL Fluor® Red 590
- Calcein
- Cy2™
- Cy3.5™
- Cy3™
- Cy5™
- DiD
- DiI
- DiO
- Draq5
- DsRed
- DyLight 488
- DyLight 549
- DyLight 649
- EGFP
- Emerald GFP
- FAM
- FITC
- Fluo-3
- Fluo-4
- FM™ 1-43
- FusionRed
- GFP
- LysoTracker Green/pH 5.2
- LysoTracker Red/pH 5.2
- LysoTracker Yellow HCK-123
- MitoTracker Deep Red 633/MeOH
- MitoTracker Green FM/MeOH
- MitoTracker Orange/MeOH
- MitoTracker Red/MeOH
- mWasabi
- Oregon Green™ 488
- Propidium Iodide
- Quasar® 570
- Quasar® 670
- R-phycoerythrin
- Rhod-2
- Rhodamine Red™-X
- ROX
- SYBR® Green I
- SYTO 9/DNA
- SYTO® 60
- TagRFP
- TAMRA
- tdTomato
- Tetramethylrhodamine isothiocyanate
- TO-PRO™-3
- TRITC
- X-rhod-1/Ca2+
- ZsGreen1
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
Filters in this Set | Price | ||
---|---|---|---|
ET470/40x (EX) | $ | 365.00 | |
ET545/25x (EX) | $ | 365.00 | |
ET640/30x (EX) | $ | 365.00 | |
89016bs (BS) | $ | 425.00 | |
ET520/40m (EM) | $ | 365.00 | |
ET595/50m (EM) | $ | 365.00 | |
ET690/50m (EM) | $ | 365.00 |
- Overview
89000
ET - Sedat Quad
- Acridine Orange + DNA
- Alexa Fluor 350™
- Alexa Fluor 405
- Alexa Fluor 488™
- Alexa Fluor 546™
- Alexa Fluor 555™
- Alexa Fluor 568™
- Alexa Fluor 647™
- Allophycocyanin (APC)
- AsRed2
- Atto 488
- Atto 550
- Atto 647N
- Azami Green
- BCECF/pH 9.0
- Biosearch Blue
- BODIPY FL/pH7.2
- Brilliant Violet™ 421
- CAL Fluor® Red 590
- Calcein
- Cy2™
- Cy3.5™
- Cy3™
- Cy5™
- DAPI
- DiD
- DiI
- DiO
- Draq5
- DsRed
- DyLight 350
- DyLight 405
- DyLight 488
- DyLight 549
- DyLight 649
- EBFP2
- EGFP
- Emerald GFP
- FAM
- FITC
- Fluo-3
- Fluo-4
- FM™ 1-43
- FusionRed
- GFP
- Hoechst 33258
- LysoTracker Blue/MeOH
- LysoTracker Green/pH 5.2
- LysoTracker Red/pH 5.2
- LysoTracker Yellow HCK-123
- MitoTracker Deep Red 633/MeOH
- MitoTracker Green FM/MeOH
- MitoTracker Orange/MeOH
- MitoTracker Red/MeOH
- mWasabi
- Oregon Green™ 488
- Pacific Blue
- Propidium Iodide
- Qdot 525
- Qdot 585
- Qdot 605
- Qdot 705
- Quasar® 570
- Quasar® 670
- R-phycoerythrin
- Rhod-2
- Rhodamine Red™-X
- ROX
- SYBR® Green I
- SYTO 9/DNA
- SYTO® 60
- T-Sapphire
- TagBFP
- TagRFP
- TAMRA
- tdTomato
- Tetramethylrhodamine isothiocyanate
- TO-PRO™-3
- TRITC
- X-rhod-1/Ca2+
- ZsGreen1
Coating: Sputter/Hard Coated
Typical Application(s): Widefield Microscopy
Microscopes: Filter cube mounting options are available when adding to cart.
Listed prices apply to:
- Round excitation and emission filters mounted in anodized aluminum rings 2.3-5.0mm thick, up to 25mm in diameter
- Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
Filters in this Set | Price | ||
---|---|---|---|
AT350/50x V2 (EX) | $ | 225.00 | |
ET402/15x (EX) | $ | 365.00 | |
ET490/20x (EX) | $ | 365.00 | |
ET555/25x (EX) | $ | 365.00 | |
ET645/30x (EX) | $ | 365.00 | |
89100bs (BS) | $ | 475.00 | |
ET455/50m (EM) | $ | 365.00 | |
ET525/36m (EM) | $ | 365.00 | |
ET605/52m (EM) | $ | 365.00 | |
ET705/72m (EM) | $ | 365.00 |
89000
NC764535 - ET - Sedat Quad
Premounted in a Leica DMi8 standard filter cube, for 25mm filters, fits 1mm-thick dichroics (91050)
Qty in Stock: 1
Filters in this Set | Price | ||
---|---|---|---|
AT350/50x V2 (EX) | $ | 225.00 | |
ET402/15x (EX) | $ | 365.00 | |
ET490/20x (EX) | $ | 365.00 | |
ET555/25x (EX) | $ | 365.00 | |
ET645/30x (EX) | $ | 365.00 | |
89100bs (BS) | $ | 475.00 | |
ET455/50m (EM) | $ | 365.00 | |
ET525/36m (EM) | $ | 365.00 | |
ET605/52m (EM) | $ | 365.00 | |
ET705/72m (EM) | $ | 365.00 | |
New Set Price | $ | 2,850.00 | |
New Cube Price | $ | 548.00 | |
New Set/Cube Cost | $ | 550.00 | |
Reclaimed Cube Cost | $ | 1,465.00 |
89000
NC1180927 - ET - Sedat Quad
Premounted in a IX3-FFXL filter cube (U-M101) for Olympus IX3 models for 32mm filters (91043)
Qty in Stock: 1
Filters in this Set | Price | ||
---|---|---|---|
AT350/50x V2 (EX) | $ | 225.00 | |
ET402/15x (EX) | $ | 365.00 | |
ET490/20x (EX) | $ | 365.00 | |
ET555/25x (EX) | $ | 365.00 | |
ET645/30x (EX) | $ | 365.00 | |
89100bs (BS) | $ | 475.00 | |
ET455/50m (EM) | $ | 365.00 | |
ET525/36m (EM) | $ | 365.00 | |
ET605/52m (EM) | $ | 365.00 | |
ET705/72m (EM) | $ | 365.00 | |
New Set Price | $ | 2,850.00 | |
New Cube Price | $ | 770.00 | |
New Set/Cube Cost | $ | 772.00 | |
Reclaimed Cube Cost | $ | 1,860.60 |
89000
NC1137472 - ET - Sedat Quad
Premounted in a BX2 filter cube (U-MF2) For Olympus BX2 and IX2 models (91018)
Qty in Stock: 1
Filters in this Set | Price | ||
---|---|---|---|
AT350/50x V2 (EX) | $ | 225.00 | |
ET402/15x (EX) | $ | 365.00 | |
ET490/20x (EX) | $ | 365.00 | |
ET555/25x (EX) | $ | 365.00 | |
ET645/30x (EX) | $ | 365.00 | |
89100bs (BS) | $ | 475.00 | |
ET455/50m (EM) | $ | 365.00 | |
ET525/36m (EM) | $ | 365.00 | |
ET605/52m (EM) | $ | 365.00 | |
ET705/72m (EM) | $ | 365.00 | |
New Set Price | $ | 2,850.00 | |
New Cube Price | $ | 578.00 | |
New Set/Cube Cost | $ | 580.00 | |
Reclaimed Cube Cost | $ | 1,495.80 |
89000
NC1084811 - ET - Sedat Quad
Premounted in a Leica DMi8 standard filter cube, for 25mm filters, fits 1mm-thick dichroics (91050)
Qty in Stock: 1
Filters in this Set | Price | ||
---|---|---|---|
AT350/50x V2 (EX) | $ | 225.00 | |
ET402/15x (EX) | $ | 365.00 | |
ET490/20x (EX) | $ | 365.00 | |
ET555/25x (EX) | $ | 365.00 | |
ET645/30x (EX) | $ | 365.00 | |
89100bs (BS) | $ | 475.00 | |
ET455/50m (EM) | $ | 365.00 | |
ET525/36m (EM) | $ | 365.00 | |
ET605/52m (EM) | $ | 365.00 | |
ET705/72m (EM) | $ | 365.00 | |
New Set Price | $ | 2,850.00 | |
New Cube Price | $ | 548.00 | |
New Set/Cube Cost | $ | 550.00 | |
Reclaimed Cube Cost | $ | 1,466.40 |
89000
NC1060760 - ET - Sedat Quad
Filters in this Set | Price | ||
---|---|---|---|
AT350/50x V2 (EX) | $ | 225.00 | |
ET402/15x (EX) | $ | 365.00 | |
ET490/20x (EX) | $ | 365.00 | |
ET555/25x (EX) | $ | 365.00 | |
ET645/30x (EX) | $ | 365.00 | |
89100bs (BS) | $ | 475.00 | |
ET455/50m (EM) | $ | 365.00 | |
ET525/36m (EM) | $ | 365.00 | |
ET605/52m (EM) | $ | 365.00 | |
ET705/72m (EM) | $ | 365.00 | |
New Set Price | $ | 2,850.00 | |
New Cube Price | $ | 490.00 | |
New Set/Cube Cost | $ | 492.00 | |
Reclaimed Cube Cost | $ | 1,428.60 |
TRF89902-EM
ET - 405/488/561/647nm Laser Quad Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greatest attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
为保证足够的平整度,TIRF所用二向色镜为2mm厚
Filters in this Set | Price | ||
---|---|---|---|
ZET405/488/561/647x (EX) | $ | 575.00 | |
ZT405/488/561/647rpc (BS) | $ | 675.00 | |
ET450/50m (EM) | $ | 365.00 | |
ET525/50m (EM) | $ | 365.00 | |
ET605/52m (EM) | $ | 365.00 | |
ET700/50m (EM) | $ | 365.00 | |
ZET405/488/561/647m (EM) | $ | 650.00 |
Mounted in a Laser TIRF for Nikon TE2000/Ti | $3,500.00 | |
Mounted in a Laser TIRF for Olympus BX2 models | $3,625.00 | |
Mounted in a Laser TIRF for Zeiss Axio | $3,375.00 | |
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters | $3,625.00 |