FRET
Förster Resonance Energy Transfer (FRET) is the transfer of photonic energy between two light-sensitive molecules (chromophores). When both chromophores are fluorophores, this is often referred to as Fluorescent Resonance Energy Transfer. The ‘donor’ molecule moves to an excited state after absorbing a photon. The ‘donor’ can then transfer this energy to the ‘acceptor’ chromophore via nonradiative dipole-dipole coupling. This energy transfer can only happen if the donor and acceptor are in close proximity to one another, making this technique very sensitive to small changes in chromophore separation. To image FRET effectively, one must clearly quantify both the donor and the acceptor emission to ensure that any FRET signal does not contain direct fluorescence from either donor or acceptor chromophores.
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- (-) Remove FRET filter FRET
- (-) Remove Texas Red® filter Texas Red®
- (-) Remove 四波段带通 filter 四波段带通
染料
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中心波长/边通波长/陷波波长
TRF69905-EM
ET - 442/514/594nm Laser Triple Band Set for TIRF applications
Set includes additional emission filters for use with external emission filter wheel for signal selection and greater attenuation of TIRF lasers.
Coating: Sputter/Hard Coated
为保证足够的平整度,TIRF所用二向色镜为2mm厚
Filters in this Set | Price | ||
---|---|---|---|
ZET445/514/594x (EX) | $ | 550.00 | |
ZT445/514/594rpc (BS) | $ | 650.00 | |
ET480/40m (EM) | $ | 365.00 | |
ET550/50m (EM) | $ | 365.00 | |
ET645/75m (EM) | $ | 365.00 | |
ZET445/514/594m (EM) | $ | 575.00 |
Mounted in a Laser TIRF for Nikon TE2000/Ti | $3,100.00 | |
Mounted in a Laser TIRF for Olympus BX2 models | $3,225.00 | |
Mounted in a Laser TIRF for Zeiss Axio | $2,975.00 | |
Mounted in a Laser TIRF for Olympus BX3/IX3 models, for 25mm filters | $3,225.00 |